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Double Fluorescent Inoculation

In reason of the constitution of its grains, the beans-caupi Vigna unguiculata (L.) Walp, Fabaceae family (Delorit and Gunn, 1986) represents a natural protein source. The raised characteristic proteinic text of its grains (about 40%) becomes the demanding culture in relation the nitrogen index required for its development. This requirement is related with the amino acid synthesis, the construction elements of proteins. The plant has its growth typically limited by the nitrogen availability an important nutrient, that acts as percussive of a series of other metabolic common ones, beyond being the main component of the molecule of clorofila (Vilarinho. the 2007). To supply this necessity, intense doses of nitrogen for the plants are required, what it can be made through the nitrogenados fertilizer job. However, when managed, efficiency of use on the part of the plant is observed low.

This if of the had one to the high losses that they occur, particularly for leaching of the ground and desnitrificao (al Hisses et., 2008). The symbiosis with caupi occurs, predominantly, with the bacteria of the Bradyrhizobium sort, characterized for the slow growth and alkaline reaction in way of culture I contend extract of leavening and manitol – YMA half (Vincent, 1970). In this simbitico event, the bacteria are attracted for the roots of the leguminosa for positive quimiotactismo due to action of flavonides set free for the plant hostess. In the interior of the nodule, the bacterium if multiplies and morphology change, forming bacterides that they are active in the setting of atmospheric nitrogen. The reduction of the concentration of oxygen in the fabric to nodular is the main signal to activate the involved genes in the setting of nitrogen and to allow that the complex of nitrogenase, sensible to the oxygen, fixes nitrogen (Dainese-hatt et al., 1999). Inside of the nodules, for the action of the enzyme nitrogenase, bacterides is capable to break the triple linking that joins two atmospheric nitrogen atoms (N2), transforming it into ammonia (NH3) (Buchanan, 2000).

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